A Microfluidic Device for Detection of Single Nucleotide Polymorphisms by Allele Specific Single Base Extension
نویسندگان
چکیده
We present a microfluidic device with integrated temperature sensors and heaters for single nucleotide polymorphism (SNP) detection. The allele specific primer is immediately annealed next to the polymorphic site, and extended by a single base. The extension product is then purified by solid phase capture, and subsequently released via chemical cleavage. After desalting, the released product is analyzed by matrix assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). Experimental results have demonstrated the ability of this microdevice to perform SNP detection, and its potential for high throughput SNP genotyping.
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